<%="课程"+int.parse(Eval("t1").ToString()).ToString()%>

------解决方案--------------------
在后台绑定时候控制，
先申明个全局变量id，绑定一次后让id++;
再找到gridview上的控件赋值给它就可以了。
如：
   
int id=1; 
  protected void grid_ItemDataBound(object sender, System.Web.UI.WebControls.DataGridItemEventArgs e)
       {
Label lbl=(Label)e.Items.FondContros["Label1"];
lbl.Text="课程："+id;
id++;
       }

------解决方案--------------------
你可以改改
C# code

string cmdstr = "select id,Uname,Upwd,CreatTime from Users";
            string connstr = @" server =**;database = ** ; uid = sa; pwd = sa";
            try
            {
                SqlDataAdapter oda = new SqlDataAdapter(cmdstr, connstr);
                DataSet ds = new DataSet();
                oda.Fill(ds, "test");
                dataGridView1.DataSource = ds.Tables[0].DefaultView;
                dataGridView1.ColumnHeadersDefaultCellStyle.Alignment = DataGridViewContentAlignment.MiddleCenter;//设置标题文本位置
                dataGridView1.DefaultCellStyle.ForeColor = Color.Blue;//设置前景色
                dataGridView1.ColumnHeadersHeightSizeMode = DataGridViewColumnHeadersHeightSizeMode.DisableResizing; //设置列标头高度
                dataGridView1.ColumnHeadersHeight = 35;//设置列标头高度
                dataGridView1.RowsDefaultCellStyle.WrapMode = DataGridViewTriState.True;//设置列文本自动换行
                dataGridView1.Columns[0].ToolTipText = "ID";
                dataGridView1.Columns[1].ToolTipText = "用户帐号";
                dataGridView1.Columns[2].ToolTipText = "用户密码";
                dataGridView1.Columns[3].ToolTipText = "创建日期";
                dataGridView1.Columns[0].HeaderCell.Value = "ID";
                dataGridView1.Columns[0].Width = Convert.ToInt16(0);
                dataGridView1.Columns[0].ReadOnly = true;
                dataGridView1.Columns[1].HeaderCell.Value = "帐 号";
                dataGridView1.Columns[1].Width = Convert.ToInt16(70);
                dataGridView1.Columns[2].HeaderCell.Value = "密 码";
                dataGridView1.Columns[2].Width = Convert.ToInt16(70);
                dataGridView1.Columns[3].HeaderCell.Value = "创建日期";
                dataGridView1.Columns[3].Width = Convert.ToInt16(100);
                dataGridView1.Columns[3].ReadOnly = true;

            }
            catch
            {
                MessageBox.Show("读取失败.....", "系统提示", MessageBoxButtons.OK, MessageBoxIcon.Error);
            }

------解决方案--------------------
                <asp:TemplateField HeaderText="userpwd">
                   <EditItemTemplate>
                       <asp:TextBox ID="TextBox3" runat="server" Text='<%# Bind("userpwd") %>'></asp:TextBox>
                   </EditItemTemplate>
                   <ItemTemplate>
                       <asp:Label ID="Label3" runat="server" Text='<%# Bind("userpwd") %>'></asp:Label>
                   </ItemTemplate>
               </asp:TemplateField>